
Insulin Production Technology
Find innovative production technology for making insulin and connect directly with world-leading specialists
Insulin is a hormone that controls blood glucose levels produced by the beta cells in the pancreas. As a medication, insulin was discovered by Frederick Banting and Charles Best in 1921, for which Banting was awarded a Nobel Prize in 1923. The insulin production process involves manipulating the biological precursor to insulin and cultivating it inside bacteria using specialized technology.
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The differences between animal-derived insulin and human insulin
Insulin injections are used for managing blood sugar levels in individuals who can’t naturally produce insulin. For many years, using insulin derived from animals, mainly porcine or bovine pancreases was the norm, but the production of human insulin became a more popular alternative since its discovery in the 1980s. Manufacturing biosynthetic human insulin via gene technologies is a preferred method for not having to depend on animals as well as the possibility to produce unlimited amounts by multiplying the insulin gene inside bacteria.
Aside from production efficiency, as extracting large amounts from animal tissue is challenging, some issues with animal-derived insulin production are related to the raw animal tissues and diseases such as Bovine Spongiform Encephalopathy (BSE) and Transmissible Spongiform Encephalopathy (TSE) and possible cross-contamination through injections. Furthermore, the chemical differences between human and animal insulin can lead to antibody attacks, inactivation and inflammation.
However, a shortage of animal-sourced insulin in developed countries is a concern for a small number of individuals who experience severe hypoglycemic episodes when using biosynthetic insulin, and generally feel healthier when undergoing an animal-derived insulin treatment.
The steps and technology in recombinant insulin production processes

Recombinant DNA is a technology for the large-scale production of insulin by inserting the human gene which carries codes for the protein insulin into a plasmid of a simple bacteria, most commonly Escherichia coli. Producers then add ligase, an enzyme that acts like glue to help the plasmid stick to the bacterium’s DNA. The bacteria undergo a fermentation process inside a large fermentation tank, where millions of bacteria replicate every 20 minutes. The insulin harvest starts by adding a mixture of lysozyme which digests the outer layer of the cell wall and a detergent mixture which separates the fatty cell wall membrane.

The insulin is separated from the bacterium’s DNA with the help of a cyanogen bromide treatment which splits protein chains at the methionine residues. An oxidizing agent is added and the mixture is placed in a centrifuge to separate cell components. The DNA mixture is purified by using one of the following techniques: ion-exchange column, reverse-phase high-performance liquid chromatography or a gel filtration chromatography column. Finally, the purified substance is ready for medical use.
The future of insulin patents and treatment delivery
Although the use of insulin pens is a practical therapy due to their reusable nature, ability to increase patient compliance and accuracy, and there are innovative solutions to enhance the delivery of insulin in the future. An alternative for diabetes care would be oral application, however, scientists are looking for ways to avoid enzymatic degradation upon consumption. Solutions can be found in nanoparticle-based approaches which protect insulin from gastrointestinal conditions and enhance the permeability of the enzyme.
A further option is the bioresponsive insulin delivery system, which accelerates insulin release with increasing glucose levels. Furthermore, manufacturers are working on the production of inhaled insulin devices, with insulin particles small enough to reach the deep lung and pass into the bloodstream. Another aerosol option is buccal insulin, a spray absorbed through the inner cheek wall. Finally, absorbing insulin through the skin with the help of insulin patches is another idea in development.

Transgenic plants as a solution for high demand
The insulin production process and its current manufacturing technologies struggle to keep up with the steadily increasing demand for affordable insulin due to a rising number of patients with diabetes. This is especially the case in the United States. One of the most effective alternatives is the manufacture of recombinant human insulin from the plant Arabidopsis Thaliana and its oilseeds. Plant-based insulin is easy to produce, cost-effective, free from human pathogens and involves high-quality protein processing.
The oilseeds are genetically engineered with the recombinant protein targeting oil bodies and then processed by separating oil bodies via liquid-liquid phase to reduce chromatography steps in the purification of insulin. Transgenic plants can produce a high level of biologically active proinsulin and it can further be enzymatically treated in vitro which is an economic method for mass production.
Processing steps involved in insulin making
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Labeling system for insulin pens
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Mini extruder for small batch processing
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Single-use bioreactor for protein expression
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Single-use fermentor for microbial fermentation
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Steam & air mixture sterilizer for parenteral solutions
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Syringe filling line for parenteral drugs
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Mobile biodecontamination unit for small to mid-size rooms
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Static autoclave for pharmaceutical sterilization
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Sanitary mixer for food and pharmaceutical processing
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High pressure homogenizers for dairy and pharmaceutical applications
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Rotary filler for pharmaceutical vials
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Production freeze dryer for pharmaceutical manufacturing
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Ready-to-use denesting equipment for pharmaceutical fill-finish
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Sterile syringe plunger placement system
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Controlled lyophilization nucleation system for drug development
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Micro-line vial filling solution for biopharmaceuticals
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Tapped density tester for pharmaceutical powders
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Automated sample preparation workstation for content uniformity testing
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Manual tablet disintegration tester
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Reactors and tanks for bioreactors and fermenters
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Benchtop tangential flow filtration system
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Triple combi filling and stoppering system for vials and ampoules
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Prefilled syringe filling and stoppering system
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Combi r&d ampoule and vial filling equipment
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Vial washing system for internal and external cleaning
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High shear mixer for efficient particle size reduction
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Automatic syringe de-nester for pharmaceutical production
Streamline your syringe production line with high-speed de-nes...

Automatic linear filling stoppering system for large volume vials
Ensure precise vial filling and secure sealing with a...

Pharmaceutical ampoule labelling system
Achieve precise and efficient labelling of ampoules with a high-speed system design...

Single-mold continuous bottle Bfs solution
Optimize aseptic production with this advanced solution, offering superior ster...

Single-use mixing system for biopharmaceutical production
Achieve precise blending and homogenization of shear-sensitive...

Surface plasmon resonance (spr) sensor chips
Enhance your molecule interactions analysis with precise sensor technology th...

Benchtop bioreactors for microbial fermentation
Optimize your bioprocessing with scalable benchtop bioreactors, ideal for ...

Stainless steel bioreactors
Enhance your bioproduction capabilities with robust solutions designed for efficient fermentatio...

Aseptic handling and fill-finish platform for Rtu syringes, vials, and cartridges
Ensure aseptic precision and high t...

High-frequency vibratory mixer
Mixing liquid products by stirring method generates heat from friction and shear forces. This...

Manual stoppering equipment for pre-filled syringes
Air present in the empty space of pre-filled syringes may prevent you...

Cold forming aluminum blister machine for capsules
Aluminum blisters are mainly used for packaging products that are sens...

Automated bubble traps for chromatography
Radial flow chromatography processes require special attention and the right use...

Pre-packed and pre-validated chromatography columns
In chromatography, you must always take into account the apparatus th...

Lab-scale radial chromatography columns for virus validation
In the food industry, as an example, during lab-testing and...

Compact H2O2 disinfection system
Dry fogging provides safe, effective microbiological cleaning of critical areas in biotech,...

Closed restricted access barrier system
Pharmaceutical production techniques often require isolation of compounds and chemi...

Disposable monolithic columns
Chromatographic monolithic columns for working with large proteins, viruses, VLPs and pDNA req...

Short bed monolithic columns for analytics
Samples taken at various process steps need to be analysed quickly and accurate...

Plasmid process pack for your pDNA purification process
When developing a pDNA purification process for eventual transfer...

Purification screening kit for large biomolecules
To purify a specific large biomolecule, for example IgM or a virus or v...

Disposable tank liners
Manufacturers in the bioprocessing industries are increasingly turning to single use solutions for the...

Flexible automatic inspection machine for ampoules, vials, cartridges
If you are looking to improve the efficiency of y...

Flexible automatic inspection machine for syringes
Manufacture of pre-filled syringes requires the highest quality inspec...

Small automatic inspector for ampoules, vials, or cartridges
Inspect small batch production of ampoules, vials, or cartr...

Semi-automatic inspection machine for ampoules, vials or cartridges
If you need to increase the efficiency of your manu...

Visual and HVLD inspection for ampoules, vials and cartridges
Do you need to improve the efficiency and capability of yo...

Single-use pressure sensor
Biopharmaceutical manufacturers are increasingly making use of Single-use pressure sensors to enh...

High-speed aseptic injectable vial filling machine
Fill up to 24,000 vials per hour with pharmaceutical powders or liquid...

Automated single use tangential flow filtration system
Tangential Flow Filtration is an essential to the biopharmaceutica...

Single-use conductivity sensor
Biopharmaceutical manufacturers are increasingly making use of single-use conductivity sensor...

Single-use temperature sensor
Biopharmaceutical manufacturers are increasingly making use of single-use temperature sensors ...

Lab scale tangential flow filtration system
Tangential Flow Filtration is an essential to the biopharmaceutical processing...

Automated cell culture clarification system
Efficient harvest from the bioreactor is essential to any successful cell cult...

Disposable tubing for manifolds in bioprocesses
Manufacturers in the bioprocessing industries are increasingly turning to ...

3D disposable bags for bioprocesses
Manufacturers in the bioprocessing industries are increasingly turning to single-use so...

Disposable manifold bags for bioprocesses
Manufacturers in the bioprocessing industries are increasingly turning to dispos...

Normal flow filtration sets for bioprocesses
Bio-processors require reliable, high quality, easy to maintain, filtration e...

Disposable mixer bags for bioprocesses
Bioprocessors must use ingredients of known high quality and consistency. Inconsist...

Disposable pillow bags for bioprocesses
Manufacturers in the bioprocessing industries are increasingly turning to single us...

Biocompatible tubing for peristaltic pumps
Sensitive bioprocess applications requires equipment that will not interfere wi...

Y sets tubing for bioprocesses
Manufactueres in the bioprocessing industries are increasingly turning to single use solution...

Semi-automated tangential flow filtration system
Tangential Flow Filtration is an essential to the biopharmaceutical proce...

Semi-automated normal flow filtration system
Normal Flow Filtration is frequently used the biopharmaceutical processing in...

Bag holder and weighing platform for bioprocesses
Single use bags for biopharmaceutical process containment and liquid st...

Lab scale normal flow filtration system
Normal Flow Filtration is frequently used the biopharmaceutical processing industry...

Automated dispensing system for bioprocesses
Biopharmaceutical manufacturers require precisely known liquid quantities for...

Superheated water shower autoclave
Specially designed for sterilizing large batches of parenteral solutions.

Dry heat depyrogenation oven
Specially designed for the drying and sterilization of glass containers, vials or ampoules, and...

Sterility test isolators
Designed to avoid this risk and protect the product from both the process and externally generated f...