High-pressure homogenizers are used in bio-pharmacy applications to obtain intracellular components like enzymes and proteins from bacteria, fungi, and animal cells through cell rupture. They are also used in other biotech processes such as the production of nanoparticles for tissue engineering and in the development of new drugs, vaccines, antibiotics, and chemicals. The choice of a high-pressure homogenizer depends on the mixture to be processed, desired particle size distribution, and the type of force to be applied to the particles.