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Cell Culture Fermentation Equipment

The fermentation process stimulates enzymatic activity in organic substrates to transform their chemical composition. Fermentation is aided by storying the biomaterials in anaerobic environments, allowing the microorganisms to decompose their nutrient structures. The process is integral to some of the most popular products throughout time such as wine, beer, cheese, and kimchi. But besides the food sector, fermentation is also an important stage in the industrial manufacturing of chemicals, pharmaceuticals, and biofuels.

Fermentation

Cell culture has been, over a little more than 100 years, an important method of research in laboratories. But medicine developments aside, we wonder if even the most positive of idealists could have also believed that cell cultures and tissue culture would possibly change the way – and what – we eat. From the lab to the dining table, there’s cell culture equipment to make even the unimaginable possible.

Cell cultures

Sensitive bioprocess applications requires equipment that will not interfere with the chemical and biological processes being carried out.  At the same time, production bioprocesses, in particular those that involve peristaltic pumping, require robust tubing not prone to wear or mechanical failures.  Traditional transfer tubing for these more rigorous ans sensitive bioprocesses often proves inadequate.


<h2>A robust biocompatible tube designed for longevity in production peristaltic pumping applications</h2>
JM BioConnect is known for certified top of industry quality and customer service including very short lead times.  The company offers tubing solutions specifically designed for use in peristaltic pumps in bioprocess production environments. The tubes are of the PharMed BPT brand and are completely bio-compatible and less permeable to gasses and vapors than traditional silicone tubes.  This makes the PharMed BPT tubes ideal for protecting sensitive biological processes like cell culture, fermentation, separation, synthesis, and purification.  At the same time, they have been formulated for enhanced flex and wear characteristics allowing trouble free used in peristaltic pumping applications.  The combination of these qualities increases the efficiency of the bio-production process by reducing down time from tubing failure without making any sacrifices in bio compatibility.

Biocompatible tubing for peristaltic pumps

In laboratory settings, producing reliable and repeatable results necessitates precise control over microbial and cell cultures. The challenge lies in minimizing contamination risks while maintaining the integrity and sterility of the cultures under study.
<h2>Facilitates precise control and sterility</h2>
The autoclavable and in-situ sterilizable lab-scale fermentors and bioreactors from Bioengineering are designed to ensure maximum sterility and control in laboratory and pilot-scale applications. These systems, ranging from benchtop to pilot scale, utilize the advanced BioSCADA software, which provides control comparable to full-scale production plants. The bioreactors are constructed with stainless steel, featuring a double shell and double floor for optimal temperature control and efficient sterilization. The belt drive system reduces vessel height, offering convenient access for all operations. Options are available for both cell culture and bacteria fermentation, including stirrer systems and gassing technologies tailored to specific requirements. Processing capacities range from 28 to 650 liters, allowing scalability from research and development to small-batch production. Complete GMP documentation ensures regulatory compliance.

Autoclavable and in-situ sterilizable lab-scale fermentors

In cell culture applications, ensuring optimal growth conditions for cells is critical. Challenges often include dealing with shear-sensitive cells, heat-instable media, and limited clean-room space. Addressing these issues requires advanced bioreactor solutions tailored to specific cell culture requirements.
<h2>Cultivate various cell cultures with precision</h2>
The bioreactors from Bioengineering AG are designed to address the specific needs of diverse cell culture processes. With capacities ranging from 30 to 25,000 liters, they offer solutions for mammalian, plant, insect cells, and virus cultivation on cells. Featuring systems for both suspended cells and adherent cells on micro-carriers, Bioengineering's bioreactors ensure precise control of environmental conditions. Special components and designs, such as gas-mixing stations, surface gassing, and bubble-free gassing, cater to shear-sensitive cells and ensure effective aeration. The perfusion systems, offering continuous production with internal or external spin filters, further enhance their efficiency. These bioreactors also integrate auxiliary vessels for metering solutions, reducing clean-room costs, and come equipped with super skids for faster installation and qualification times.

Bioreactors for cell culture processes

When conducting fermentation research, ensuring the sterility of the cultivable environment is crucial. Researchers face challenges in maintaining consistent temperature, pH, and oxygen levels for optimal microbial or cell growth.
<h2>Optimize microbial growth in sterile conditions</h2>
The BRS Biotechnology Equipment autoclavable lab fermentors and bioreactors are designed for research applications and fermentation process optimization. These fermentors, available in volumes ranging from 1 to 10 liters, feature table-mounted design for ease of maintenance and operation. Connections for water and air are accessible, reducing setup time. Measurement and control tools are housed in a separate cabinet for easy access and customization. They are suitable for both batch and continuous cultivation of bacteria, fungi, yeasts, and cells, and for small-scale production of proteins and monoclonal antibodies. Made from 316L steel and borosilicate glass, these fermentors ensure durability and resistance to contamination. Control functions include temperature, agitation speed, pH, and dissolved oxygen monitoring, with advanced options for multi-channel feeding and exhaust analysis.

Autoclavable glass fermentor for biotech research

Scaling up microbiological synthesis processes from the laboratory to small-scale production requires precise and reliable equipment. This is vital for applications in pharmaceutical production, veterinary chemicals, and biotechnology research centers.
<h2>Facilitates efficient scale-up and small-scale production</h2>
The BRS pilot fermentors and bioreactors by Brs Biotechnology Equipment serve as a crucial link between laboratory-scale experiments and larger production scales. These units, with volumes ranging from 5 to 80 liters, are designed for small-scale pilot production of agricultural and veterinary chemicals, pharmaceutical production, and diverse biotech processes. Their platform-mounted, open-frame modular construction allows easy access for maintenance and operations. Central connections for steam, water, and air minimize start-up time and effort. Advanced models feature automatic air flow metering, optical density sensors, and remote control systems via SCADA. The fermentors support a wide range of processes involving enzymes, bacteria, fungi, algae, and various cell cultures. Compliant with GMP and FDA standards, they are optimized for medium and process optimization in research settings.

Pilot fermentors for biotech processes

In process development for cell culture and microbial applications, conserving valuable materials and achieving precise control over experimental conditions are critical challenges. This is particularly relevant in scenarios involving small working volumes and the need for parallel processing capabilities.
<h2>Efficiently develop cell culture and microbial processes</h2>
The DASbox Mini Bioreactor from Eppendorf is an industry-standard autoclavable glass bioreactor designed for use in cell culture and microbial process development. With working volumes of 60–250 mL, it is ideal for conserving resources while ensuring scalability and reproducibility. The bioreactor features a stainless steel head plate equipped with standard sensors for precise measurement and control of temperature, pH, dissolved oxygen (DO), and level. It also comes with a powerful direct overhead drive, configured with either a marine impeller for cell cultures or two Rushton-type impellers for microbial applications. Additionally, the liquid-free exhaust condenser offers automatic slide-in activation and slide-out deactivation for easy handling.

Mini bioreactor for cell culture and microbial process development

When working with microbial applications like bacterial, yeast, or fungal cultures, maintaining high cell density and ensuring effective mass transfer are vital. Traditional bioreactors often require extensive cleaning and sterilization, posing significant work interruptions and potential contamination risks.
<h2>Optimized for small-scale microbial fermentation</h2>
The BioBLU® f Single-Use Bioreactors by Eppendorf are rigid-walled stirred tank bioreactors designed specifically for robust microbial applications. These bioreactors mimic traditional bioreactors in their design, making them compatible with established laboratory processes. The BioBLU® f series covers a working volume range from 65 mL to 3.75 L, supporting high cell density fermentation. This series incorporates multiple Rushton-type impellers for efficient mixing and mass transfer. The interior baffles enhance mixing and provide effective heat removal, vital for high-density fermentation. The bioreactors come preassembled with spargers, gas filters for inlet and exhaust, and are sensor-ready for pH, dissolved oxygen (DO), and temperature probes. Various head plate ports allow for flexibility in additional fittings. BioBLU 0.3f and 1f models are delivered sterile, irradiated by >15 kGy (β) or >25 kGy (X-ray). The BioBLU 3f model can be sterilized using an autoclave, offering further flexibility. Suitable for integration with Eppendorf bench-scale bioprocess controllers and select third-party controllers, BioBLU® f bioreactors minimize the risks of contamination and downtime typically associated with traditional glass bioreactors.

Single-use bioreactors for microbial fermentation

In the field of bioprocessing, developing and scaling up microbial and cell cultures often requires precise, scalable, and contamination-free systems. Ensuring reliable and reproducible results while maintaining process control is crucial for vaccine and medicine production.
<h2>Customizable for varied bioprocessing needs</h2>
The Applikon BioBench by Getinge is a modular bioreactor specifically designed for bench-top bioprocessing applications. Its stainless steel construction and Sterilize-in-Place capability eliminate the risk of contamination from external factors. The system retains geometric ratios consistent with commercial-scale bioreactors, allowing for accurate scale-up studies. It includes multiple configuration options such as porous spargers, water-cooled exhaust gas condensers, and various impeller types, making it highly adaptable to specific process requirements. Suitable for both microbial and cell cultures, the bioreactor offers a compact design that saves floor space and meets cGMP and GAMP compliance requirements, simplifying validation processes.

Bench top bioreactor for microbial and cell cultures

In a research laboratory setting, managing multiple components for bioprocessing can be time-consuming and error-prone. Efficient and standardized solutions are needed to streamline cell culturing processes and ensure reproducibility across experiments.
<h2>Facilitates efficient and reproducible bioprocessing</h2>
The Applikon BioBundle by Getinge is a complete bioreactor system designed for ease of use and sophisticated control. The system arrives with all necessary components, including silicone tubing and sample bottles, making it ready for immediate use. Its intelligent and powerful process controller ensures accurate management of bioprocess parameters and actuators through both digital and analog outputs. The system's advanced autotuning adaptive control minimizes the need for manual PID controller settings, improving process accuracy. Additionally, the compact design integrates essential accessories into a single console, optimizing lab space. This solution supports various applications including cell cultures, microbial cultures, batch, fed-batch, and continuous bioprocessing.

Turnkey bioreactor system for cell culture

Scaling up from lab-scale bioreactors to small-scale production requires a solution that ensures consistent and efficient transfer of processes. Without such technology, delays in production can result from challenges in customization and validation.
<h2>Streamline upstream bioprocessing</h2>
The Applikon BioPilot from Getinge is a pilot plant stainless steel bioreactor designed for upstream bioprocessing. This bioreactor is modular, using standard modules to facilitate customization for both microbial and cell cultures. It features a Sterilize-in-Place (SIP) system and a compact design that saves floor space. The bioreactor is constructed from pharma-grade 316L stainless steel with a surface finish of Ra < 0.4 micron, ensuring ease of cleaning and reducing contamination risks. The Applikon BioPilot's magnetically coupled agitator and consistent component scaling from lab-scale simplify validation and minimize human error. It complies with cGMP and GAMP standards, further easing the process of validation. This bioreactor is ideal for various applications, including scale-up studies and process optimization. The available volume capacities range from 30 liters to 270 liters, with options for microbial or cell culture operations.

Stainless steel bioreactor for small-scale bioprocess production

Managing microbial and cell cultures in a lab environment often requires precise control over conditions while also conserving space and resources. In such environments, achieving scalable results with minimal media usage is crucial.
<h2>Facilitate small-scale bioprocessing</h2>
The Applikon MiniBio by Getinge is a compact, multi-use glass autoclavable bioreactor designed for small-scale operations in research laboratories. It allows for precise control of microbial and cell cultures while conserving bench space and reducing media costs. Its small volume is ideal for screening studies, media optimization, and process optimization. The bioreactor features a direct drive system with lipseals, choice of Rushton or Marine impellers, and electrically cooled exhaust gas condenser. It also includes configurable Luer fittings for multiple additions, sensors, and fittings. Available in three sizes—250 mL, 500 mL, and 1000 mL—the MiniBio is suitable for batch, fed-batch, perfusion, and continuous cultivation processes.

Small size multi-use glass autoclavable bioreactor

In research laboratories, managing multiple bioreactors can present challenges in maintaining precise control over experimental conditions. This often results in inefficiencies and delays in research and development processes.
<h2>Configure and control multiple bioreactors simultaneously</h2>
The Livit Flex from Getinge is an advanced bioprocess controller designed for lab-scale bioreactors. It can be configured as a single or dual control system to optimize bench space and streamline workflow in your laboratory. Compatible with both single-use and multi-use bioreactors, the Livit Flex enables seamless integration and efficient data management. It features a user-friendly interface with a 13.3-inch LCD touchscreen and intuitive Livit software platform, which allows easy access and modification of all settings. The Livit Flex supports a variety of sensor options and auxiliary devices like external pumps, ensuring automatic recognition and quick setup. Ideal for microbial and cell culture applications, this controller is designed to reduce operator error and enhance experimental productivity.

Controller for lab-scale bioreactors

Some of the vaccines in use today were originally developed decades ago. But advances in research, expertise and vaccine development equipment provide immunizations from bacterial and viral diseases (antibodies and cell therapy) to more people every year. The Covid 19 pandemic highlights the urgent need to scale up the production of vaccines and ensure an automated as well as reproducible manufacturing environment.

Vaccines

Getinge Applikon is the only company that can take you from the initial screening stage to full-scale production using the same platform. This minimizes scale-up risks and guarantees the shortest time to market for your new product development.

How can single use in biotech be sustainable, if you think of all the waste it creates? This might raise questions regarding the environmental impact. Let’s compare the complete life cycle of single-use systems such as tubing, capsule filters, chromatography devices, and bioreactors to their multi-use counterparts in glass or stainless steel and look at the outcome.

<h2>The end-of-life impact of single use tech</h2>
A product life cycle assessment compared single-use technologies to traditional multi use systems on points like resource extraction, processing, manufacturing, all through the end of the product life. Additionally environmental impact factors such as carbon, energy and water footprint were evaluated to paint a complete sustainable picture. Although disposal and waste remains a concern, the study found that this was actually only 1% of the total impact on the environment. Once put into use single use equipment presented a significantly lower impact resulting in <a href="https://cdn.cytivalifesciences.com/dmm3bwsv3/AssetStream.aspx?mediaformatid=10061&amp;destinationid=10016&amp;assetid=16801">a smaller overall end-of-life impact</a>.
<h2>Single use eliminates the need for cleaning in between batches</h2>
The most pressing finding is that the impact of chemicals, high purity waters and heat for cleaning and sterilization in between production batches is very high. Take for example a <a href="https://making.com/equipment/single-use-bioreactor">single use bioreactor</a> as shown in the video below; No need for extensive cleaning before the culture is started or after it’s finished and reducing the risk of cross contamination. Innovative technology such as 3D printing allows to create customizable products, while (re)creating exact replicas and keep desired process environments steady. Now officially stated as the more sustainable choice makes us understand why single systems are on the rise.

[embed]https://www.youtube.com/watch?v=RfPgrgw_wT8[/embed]

Is single use biotech equipment the more sustainable option?

Pharmaceutical

Biopharmaceuticals are drugs in which the active substance is manufactured or extracted from biological origin materials. They are usually produced in bioreactors by plant cells, microbial cells, yeast cultures, or mammalian cells. Also known as biomanufacturing, they are used to either treat or diagnose and prevent a patient’s medical condition or disease. Biopharmaceuticals are typically complex macromolecules administered by injection or infusions, and examples include vaccines, serums, and insulin.

Biopharmaceuticals

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