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Cell Culture Separation Equipment

Industrial separation equipment encapsulates the separation process which is to separate components of a liquid mixture, for recovering dissolved solids from liquids. The choice of a suitable technology depends on the identification of the key physical or chemical property, such as size, mass, density, or boiling point, which sets the product of interest apart from the rest of the components. Separation technology is widely used in the pharmaceutical and chemical industries for the isolation of active ingredients and high-purity chemicals. As well as in waste management and recycling industries.

Separation

Cell culture has been, over a little more than 100 years, an important method of research in laboratories. But medicine developments aside, we wonder if even the most positive of idealists could have also believed that cell cultures and tissue culture would possibly change the way – and what – we eat. From the lab to the dining table, there’s cell culture equipment to make even the unimaginable possible.

Cell cultures

Chromatographic monolithic columns for working with large proteins, viruses, VLPs and pDNA require the high structural strength and contamination protection usually provided by expensive medical grade stainless steel.
<h2>Separate complex biomolecules using strong, versatile multi-use columns</h2>
CIMmultus™ disposable monolithic columns from BIA Separations have the structural integrity and leachable profile of medical grade stainless steel, but the process economics of a single or multi-use device.

With the introduction of composite materials into an epoxy-based housing and the application of USP Class VI Parylene C coating to the entire column surface, CIMmultus™ columns offer safety and high performance.

CIMmultus™ columns can be used at any step in the separation process and, with their 2 µm flow-through channels allowing convective mass transfer, make a perfect choice for the purification of viruses, VLPs, plasmids, antibodies, PEGylated proteins and other large biomolecules.

Disposable monolithic columns

Samples taken at various process steps need to be analysed quickly and accurate results delivered, without affecting the state or condition of the original sample.
<h2>Understand a bioprocess through real-time monitoring</h2>
CIMac™ Analytical Columns from BIA Separations are short bed, high performance monolithic columns offering all the advantages of CIM® monolithic technology. They are primarily intended for fast, efficient, and reproducible separations of biomolecules like large proteins, plasmid DNA, and viral particles.

Small volume and short column length allow operation at high volumetric flow rates (from 1 to 30 column volumes/min), enabling product quantity and purity information to be available in just a few minutes.

The CIMac™ Analytical Columns are pre-packed in dedicated stainless steel housings and allow user-friendly connections to HPLC equipment, hence they can be effectively used for both the in-process and final control of various samples from different purification processes.

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Short bed monolithic columns for analytics

When developing a pDNA purification process for eventual transfer into production, efficient examination and selection of protocols is critical.
<h2>Develop plasmid purification procedures quickly</h2>
BIA Separations' CIMmultus™ HiP2 Plasmid Process Pack™ is intended to help scientists easily develop their pDNA purification process and transfer it to production.

Allowing developers to select only the relevant parts of the recommended protocol that best fits their needs, the pack saves time and money on all scales.

The CIMmultus™ HiP2 Plasmid Process Pack™ contains one weak anion-exchange (DEAE) and one hydrophobic (C4 HLD) column and a protocol describing the purification procedure of specific plasmids.

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Plasmid process pack for your pDNA purification process

To purify a specific large biomolecule, for example IgM or a virus or virus-like particle, the first step is to screen the working conditions. Different mobile phases and column chemistries are then tested, according to the biochemical properties of that particular large molecule.
<h2>Efficiently identify, isolate and recover large biomolecules</h2>
The CIMmultus™ Purification Screening Kit from BIA Separations is intended to help scientists quickly discover the optimal stationary phase chemistry for their particular application.

The kit includes two different 1mL CIMmultus™ ion-exchange columns (QA and SO3) and one hydrophobic interaction chemistry column (OH).

By using one of the selected chemistries and applying proper working conditions, the user will be able to discover the combination that will result in appropriate purity and excellent recovery of their required large biomolecule.

Purification screening kit for large biomolecules

In biopharmaceutical production, ensuring the integrity of cell cultures during the separation process is crucial. Traditional separation methods can cause cell damage, leading to reduced yields and increased downstream processing challenges.
<h2>Gentle cell harvesting and efficient protein recovery</h2>
The Alfa Laval Culturefuge is a range of bottom-fed hermetic cell centrifuges designed for the gentle harvesting of mammalian cell cultures, microbial cells, and precipitated proteins. What makes the Culturefuge unique is its hollow spindle feed mechanism, which ensures gentle acceleration of the feed liquid to rotational speed, thereby minimizing cell lysis and preventing the release of unwanted intracellular proteins. This results in enhanced yields and optimized downstream operations. Offered in skid-mounted configurations, the Culturefuge systems include comprehensive features like an integrated electrical system with PLC, pneumatic unit, and piping for service and process liquids. They meet major pressure vessel specifications such as ASME and PED. Available in configurations suitable for capacities ranging from 200 to 20,000 liters per hour, the Culturefuge series can scale from pilot plants to full production. Additional options include superfine electro-polished contact surfaces and steam-sterilizable pumps for solid transport.

Biopharma cell centrifuge for gentle protein recovery

In the pharmaceutical industry, producing vaccines and other biopharmaceutical products often involves clarifying microbial cells, cell cultures, or precipitated proteins. This process is critical for product yield and involves minimizing shear stress and preventing contamination.
<h2>Efficiently harvest microbial cells</h2>
The Alfa Laval MBPX range of high-speed separators is specifically designed for the pharmaceutical industry and microbiological separation applications. These separators handle suspended particles typically sized 0.5 – 500µm with high efficiency due to their gentle processing. They are designed to minimize shear stress, eliminate oxygen presence, consume low power, and operate quietly. MBPX separators can be customized as fully automated systems and are available as skid-mounted solutions for smaller sizes or modular systems for larger scales, including all necessary components for service and process liquids and electrical systems. They meet high hygiene demands with fully hermetic designs that enable gentle, foam-free processing, and ensure high product yield with minimal losses. They also fulfill ASME BPE standards, CFR part 11, and USP class VI standards for wetted polymers and seal rings.

Microbial cell clarification solution

In biopharmaceutical manufacturing, the integration of various systems for upstream and downstream processes such as cell culture and purification is crucial to maintain efficiency and product quality. Customizable solutions that cater to specific process needs are often required to meet stringent regulatory standards and production goals.
<h2>Integrate stainless steel and single-use systems</h2>
The Hybrid Bioprocess System from Austar offers a comprehensive solution incorporating both stainless steel and single-use systems for upstream and downstream bioprocessing. Designed to handle tasks such as media preparation, buffer preparation, cell culture, separation, purification, and aseptic transfer, this system ensures flexibility and customization according to client needs. By combining the technical benefits and cost advantages of stainless steel with single-use systems, it provides high-quality, low-cost solutions. The system includes hardware, consumables, automatic control, and validation services. Additionally, it supports the entire process from feasibility evaluation to project management services, making it a versatile option for biopharma operations.

Hybrid bioprocess system for cell culture and purification

Some of the vaccines in use today were originally developed decades ago. But advances in research, expertise and vaccine development equipment provide immunizations from bacterial and viral diseases (antibodies and cell therapy) to more people every year. The Covid 19 pandemic highlights the urgent need to scale up the production of vaccines and ensure an automated as well as reproducible manufacturing environment.

Vaccines

Getinge Applikon is the only company that can take you from the initial screening stage to full-scale production using the same platform. This minimizes scale-up risks and guarantees the shortest time to market for your new product development.

How can single use in biotech be sustainable, if you think of all the waste it creates? This might raise questions regarding the environmental impact. Let’s compare the complete life cycle of single-use systems such as tubing, capsule filters, chromatography devices, and bioreactors to their multi-use counterparts in glass or stainless steel and look at the outcome.

<h2>The end-of-life impact of single use tech</h2>
A product life cycle assessment compared single-use technologies to traditional multi use systems on points like resource extraction, processing, manufacturing, all through the end of the product life. Additionally environmental impact factors such as carbon, energy and water footprint were evaluated to paint a complete sustainable picture. Although disposal and waste remains a concern, the study found that this was actually only 1% of the total impact on the environment. Once put into use single use equipment presented a significantly lower impact resulting in <a href="https://cdn.cytivalifesciences.com/dmm3bwsv3/AssetStream.aspx?mediaformatid=10061&amp;destinationid=10016&amp;assetid=16801">a smaller overall end-of-life impact</a>.
<h2>Single use eliminates the need for cleaning in between batches</h2>
The most pressing finding is that the impact of chemicals, high purity waters and heat for cleaning and sterilization in between production batches is very high. Take for example a <a href="https://making.com/equipment/single-use-bioreactor">single use bioreactor</a> as shown in the video below; No need for extensive cleaning before the culture is started or after it’s finished and reducing the risk of cross contamination. Innovative technology such as 3D printing allows to create customizable products, while (re)creating exact replicas and keep desired process environments steady. Now officially stated as the more sustainable choice makes us understand why single systems are on the rise.

[embed]https://www.youtube.com/watch?v=RfPgrgw_wT8[/embed]

Is single use biotech equipment the more sustainable option?

Pharmaceutical

Biopharmaceuticals are drugs in which the active substance is manufactured or extracted from biological origin materials. They are usually produced in bioreactors by plant cells, microbial cells, yeast cultures, or mammalian cells. Also known as biomanufacturing, they are used to either treat or diagnose and prevent a patient’s medical condition or disease. Biopharmaceuticals are typically complex macromolecules administered by injection or infusions, and examples include vaccines, serums, and insulin.

Biopharmaceuticals

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