Single-use bioreactor for cell culture applications

Achieve optimal cell growth and product yield with a single-use bioreactor designed to streamline scaling from pilot studies to commercial production.

Facilitates Mixing, Sparging, and CO2 Stripping

The HyPerforma™ 5:1 Single-Use Bioreactor from Thermo Scientific is designed for biopharmaceutical manufacturers seeking reliable scalability. It utilizes a water-jacketed system with an AC motor for precise control, enabling effective mixing and sparging essential for high-quality protein expression and viral vector manufacturing. With sizes ranging from 50 to 2000 liters, the HyPerforma™ S.U.B. ensures consistent scalability across pilot-scale to commercial production. Its unique mixing system maintains sterility and minimizes foam damage, enhancing productivity by reducing the need for multiple vessels. Integrating seamlessly with Finesse™ or Applikon™ control systems, it supports diverse bioproduction environments using software platforms like Delta V™, Allen-Bradley™, or Siemens™. The single-use design includes load cells for precise monitoring and compatibility with industry-standard sensors for pH, DO, and pressure, meeting GMP and FDA standards. Its minimized footprint and load cells as standard features optimize operational efficiency, while optional component customization provides flexibility to meet specific process requirements.

Benefits

  • Enhances productivity by enabling efficient scale-up from pilot to commercial production.
  • Minimizes operational costs with its compact design and reduced vessel count.
  • Ensures consistent product quality through precise control of mixing and sparging processes.
  • Facilitates seamless integration with leading control systems for improved process management.
  • Complies with GMP standards, supporting regulatory requirements in biopharmaceutical production.

General


Applications
Cell culture,Viral vector manufacturing,Cell therapy,Biopharmaceuticals,Gene therapy,Protein expression,Vaccine production
End products
Cho-derived proteins,Crispr-modified cells,Lentivirus vectors,Monoclonal antibodies,Covid-19 vaccines,Adenovirus vectors,Car t-cells
Steps before
Cell inoculation,Media preparation,Sterilization preparation
Steps after
Harvesting,Purification,Filtration,Downstream processing
Input ingredients
cell culture media,microcarriers,suspension cells,gas (for sparging)
Output ingredients
protein products,cultured cells,cellular biomass
Market info
Thermo Fisher Scientific is renowned for providing innovative scientific instruments, laboratory equipment, and engineered-to-order solutions across various industries, esteemed for their high-quality products and expertise in life sciences, pharmaceuticals, and biotechnology sectors.

Technical Specifications


Unit Size
50,100,250,500,1000,2000 L
Mixing System
Proprietary overhead mixing
Motor Type
AC Motor
Turn-Down Ratio
5
Sparger Technology
Patented cross-flow sparger
Automation
Precise agitation control
Load Cells
Standard feature,analog or digital communication
Sensing Options
Single-use and traditional sensors for pH,DO,pressure
Application
Batch,fed batch,perfusion,suspension,microcarrier cultures
Control System
Standalone or turnkey system with Finesse™/Applikon™
Software Platforms
PC,Delta V™,Allen-Bradley™,Siemens™
Material
Stainless Steel

Operating Characteristics


Mixing system
Conventional overhead mixing
Sterility and integrity management
Single-use sterile contact surfaces
Automation level
Manual / PLC / SCADA
CIP/SIP
Manual / Thermo Fisher systems
Cleaning method
SIP / Manual
Batch vs. continuous operation
Batch
Gas introduction method
Patented cross-flow sparger technology
Mass transfer efficiency
Efficient CO2 stripping

Material Compatibility


Biological compatibility
Yes
Abrasion resistance
High
Corrosive resistance (e.g. acids)
Yes
Cleanability
Easy
Density/particle size
0.5–2.5 g/cm³ / 50–1000 µm

Physical Characteristics


Machine footprint
Minimized vessel footprint
Discharge method
Batch,fed batch,and perfusion cultures
Tank shape and size
50,100,250,500,1000,and 2000 L sizes
Compact footprint
Yes
Control panel type
PC,Delta V™,Allen-Bradley™,or Siemens™ software platforms
Optional features
Cable management tree,Industry-leading single-use and traditional sensors,Stand-alone or complete system
Material
Stainless Steel
Mixing system
Unique proprietary mixing system with overhead mixing

Custom Options


Control panel type
Finesse™ or Applikon™ control systems
Integration possibilities
PC,Delta V™,Allen-Bradley™,Siemens™ software platforms
Motor type
AC or DC motors
Cable management
Optional cable management tree
Sensor compatibility
Single-use and traditional sensors for pH,DO,pressure