Precast agarose gels for Dna electrophoresis
Achieve precise DNA separation and purification effortlessly with bufferless precast agarose gels, designed to enhance efficiency in your molecular workflows while reducing preparation time and improving safety.
Runs DNA Electrophoresis and Enhances Cloning Efficiency
The E-Gel Precast Agarose Gels from Thermo Fisher Scientific offer a robust solution for DNA electrophoresis. These gels are specially designed to be bufferless with embedded electrodes, simplifying the workflow by eliminating gel preparation and additional buffers. Ideal for molecular biology and genomics applications, they support processes such as genotyping assays, DNA fragment size selection, and high-efficiency cloning. A diverse range of agarose percentages and well formats caters to both low and high-throughput operations, ensuring adaptability across various research and diagnostic settings.
Incorporating an ion-generating system and SYBR Safe DNA stain, these gels provide safer, rapid electrophoresis without chemical staining, detecting as little as 2–3 ng of DNA within 30 minutes. Suitable for integration with high-throughput systems, they enhance productivity in laboratories by accommodating up to 96 samples simultaneously. The E-Gel CloneWell II variant mitigates UV damage and improves cloning efficiency by allowing direct retrieval of migrated DNA bands. Customizable agarose percentages and staining options ensure compliance with diverse experimental needs and industry regulations.
Benefits
- Streamlines workflow by eliminating gel preparation and additional buffers.
- Enhances safety with SYBR Safe DNA stain, reducing exposure to ethidium bromide.
- Supports high-throughput analysis with up to 96 samples, boosting lab productivity.
- Increases cloning efficiency by preventing UV damage during DNA band retrieval.
- Provides versatile application with customizable agarose concentrations and well formats.
- Applications
- Plasmid preparations,Pcr products,Molecular biology applications,Dna electrophoresis,Restriction digests,Cloning efficiency,Ngs library preparation
- End products
- Gene expression profiles,Crispr-cas9 gene editing vectors,Amplified dna fragments,Rna-seq libraries,Genetic linkage maps,Genotyping assays,Mutagenesis constructs,Dna sequencing libraries,Recombinant plasmids
- Steps before
- DNA sample preparation,PCR amplification,Plasmid preparation,Restriction digestion
- Steps after
- DNA fragment analysis,Cloning,NGS library preparation,DNA purification
- Input ingredients
- sample DNA,PCR products,plasmid preparations,restriction digests
- Output ingredients
- electrophoresis results,purified DNA fragments,DNA bands,DNA ladder comparisons
- Market info
- Thermo Fisher Scientific is renowned for providing innovative scientific instruments, laboratory equipment, and engineered-to-order solutions across various industries, esteemed for their high-quality products and expertise in life sciences, pharmaceuticals, and biotechnology sectors.
- Run time
- 8–40 min
- Agarose Percentage
- 0.8%,1.0%,2.0%,4.0%
- Resolution
- 10 bp–10 kb
- Sample Volume
- 15–25 µL
- Stain
- SYBR Safe DNA stain or Ethidium bromide
- Well Format
- Single row of 11 wells,two rows of 7 wells,48 sample wells,96 sample wells
- Electric Field
- Sustained electric field via ion exchange matrices
- Working mechanism
- Bufferless with embedded electrodes
- Integrated steps
- Precast gel with embedded ion exchange matrices
- Automation level
- Automated gel electrophoresis with E-Gel system
- Batch vs. continuous operation
- Batch
- Cleaning method
- Not applicable
- Sample throughput
- Low and high throughput options
- Safety features
- SYBR Safe DNA stain for safer analysis
- Biological compatibility
- SYBR Safe DNA stain
- Cleanability
- No manual cleaning required
- Corrosive resistance (e.g. acids)
- Compatible with electrophoresis buffers
- Density/particle size
- Not applicable
- Application Type
- Cloning,Library Preparation,Electrophoresis
- Gel size
- 48-well / 96-well
- Cassette size
- Transparent plastic cassette
- Electrode Integration
- Embedded in agarose matrix
- Ion exchange matrices
- Integrated for sustained electric field
- Well formats
- Single row,double comb,48-well,96-well
- Agarose percentage
- 0.8%,1.0%,2.0%,4.0%
- Stain type
- SYBR Safe DNA stain,SYBR Gold stain,Ethidium bromide
- Sample throughput capacity
- Low throughput,high throughput
- Electrophoresis goal
- General purpose,cloning,NGS library prep