High throughput protein analysis system
Enhance your laboratory’s capability to perform high-throughput kinetic and quantitation analysis with fluidics-free precision, ideal for advancing biologics research and production efficiency.
Performs High-Throughput Kinetic and Quantitation Analyses
The Octet® R8 Protein Analysis System from Sartorius leverages biolayer interferometry for high-throughput, label-free biomolecular interaction analysis. Its fluidics-free design minimizes maintenance and allows for non-destructive testing, streamlining assays in R&D and quality control. With eight independent channels, it performs kinetic and quantitation analyses across a range of applications such as monoclonal antibody development and mRNA vaccine production. Capable of handling up to 96 samples in under 3 hours, it supports unattended, parallel processing with integrated sample cooling for temperature-sensitive assays. The system is GxP-ready, offering 21 CFR Part 11 compliance features for regulated environments. Adaptable for various biomolecules, it fits seamlessly into biopharmaceutical and life sciences workflows, providing precise real-time kinetic and affinity data critical for decision-making in drug discovery and manufacturing.
Benefits
- Enhances productivity with simultaneous analysis of up to 96 samples.
- Minimizes maintenance and conserves samples through fluidics-free operation.
- Supports regulatory compliance with GxP-ready features and 21 CFR Part 11 software.
- Increases data accuracy and reliability with real-time kinetic and affinity characterization.
- Flexible throughput options accommodate a wide range of biomolecule sizes and types.
- Applications
- Immunology,Immuno-oncology,Vaccines,Functional profiling,Monoclonal antibodies,Biosimilars,Mrna production,Regenerative medicine,Biopharmaceuticals,Blood and plasma,Oncology,Cell and gene therapy,Quality control,Infectious diseases,Bioprocessing
- End products
- Biologics,Biosimilar rituximab,Her2 targeted therapies,Anti-pd-1 monoclonal antibodies,Chemotherapeutic agents,Aav-based gene therapies,Mrna vaccines,Plasma-derived proteins,Influenza vaccines,Diagnostic kits,Car t-cell therapies,Regenerative skin grafts,Igg antibodies,Fc fusion proteins,Sars-cov-2 neutralizing antibodies
- Steps before
- Sample Preparation,Crude Sample Collection,Reagent Qualification,Method Development
- Steps after
- Data Analysis,Quality Control,Validation,Reporting,Sample Recovery
- Input ingredients
- proteins,antibodies,peptides,DNA,RNA,liposomes,viruses,virus-like particles (VLPs),serum,buffers containing DMSO,periplasmic fractions,untreated cell culture supernatants,crude cell lysates
- Output ingredients
- real-time kinetic binding sensorgrams,fitted result plots,residuals of fits,calibration curves,tabulated concentration data,tabulated kinetic data,epitope binning matrices,cross-blocking matrices,trace overlays
- Market info
- Sartorius AG is renowned for manufacturing high-precision laboratory and bioprocess equipment. They specialize in filtration, fermentation, and cell cultivation systems, known for their innovation and reliability in the pharmaceutical and biotech industries.
- Number of Channels
- 8 independent channels
- Simultaneous Sample Analysis
- Up to 96 samples
- Data Collection Rate
- 2,5 or 10 Hz
- Sample Volume
- 180–220 µL/well
- Temperature Control
- 15°C to 40°C
- Spectrometers
- 8
- Automation
- GxP compliant options
- Detection Technology
- Bio-Layer Interferometry (BLI)
- Molecular Weight Detection Limit
- >150 Da
- Association Rate Constant (ka)
- 10^1 to 10^7 M^-1s^-1
- Dissociation Rate Constant (kd)
- 10^-6 to 10^-1 s^-1
- Affinity Constant (KD)
- 1 mM – 10 pM
- Baseline Noise (RMS)
- <3.0 pm
- Baseline Drift
- <0.12 nm/hour
- Instrument Weight
- 72 lb (32.7 Kg)
- Instrument Dimensions (H x W x D)
- 19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm)
- Analysis Time per Sample
- 5 minutes to 4 hours
- Quantitation Time for Human IgG
- 2 minutes for 8 samples
- Direct Quantitation Range
- 0.05 – 2000 μg/mL
- Automation level
- Manual / PLC / SCADA
- Batch vs. continuous operation
- Batch / Inline Continuous
- CIP/SIP
- CIP 121°C / SIP 135°C
- Energy efficiency
- 0.5–2 kWh/kg moisture
- Unattended Run Time
- Up to 12 hours
- Sample Plate Cooling
- 15°C to 40°C
- Density/particle size
- 0.5–2.5 g/cm³ / 50–1000 µm
- Machine Footprint
- 19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm)
- Weight
- 72 lb (32.7 Kg)
- Control panel type
- Optional evaporation cover for sample protection
- Integration possibilities
- GxP-compliant software integration
- Channel usage
- Eight parallel,independent channels for flexibility
- Sample plate cooling
- Temperature control from 15-40°C
- Fluidics-free design
- Minimized assay time and maintenance
- Software upgrade
- Optional 21 CFR Part 11 software