High throughput protein analysis system

Enhance your laboratory’s capability to perform high-throughput kinetic and quantitation analysis with fluidics-free precision, ideal for advancing biologics research and production efficiency.

Performs High-Throughput Kinetic and Quantitation Analyses

The Octet® R8 Protein Analysis System from Sartorius leverages biolayer interferometry for high-throughput, label-free biomolecular interaction analysis. Its fluidics-free design minimizes maintenance and allows for non-destructive testing, streamlining assays in R&D and quality control. With eight independent channels, it performs kinetic and quantitation analyses across a range of applications such as monoclonal antibody development and mRNA vaccine production. Capable of handling up to 96 samples in under 3 hours, it supports unattended, parallel processing with integrated sample cooling for temperature-sensitive assays. The system is GxP-ready, offering 21 CFR Part 11 compliance features for regulated environments. Adaptable for various biomolecules, it fits seamlessly into biopharmaceutical and life sciences workflows, providing precise real-time kinetic and affinity data critical for decision-making in drug discovery and manufacturing.

Benefits

  • Enhances productivity with simultaneous analysis of up to 96 samples.
  • Minimizes maintenance and conserves samples through fluidics-free operation.
  • Supports regulatory compliance with GxP-ready features and 21 CFR Part 11 software.
  • Increases data accuracy and reliability with real-time kinetic and affinity characterization.
  • Flexible throughput options accommodate a wide range of biomolecule sizes and types.

General


Applications
Immunology,Immuno-oncology,Vaccines,Functional profiling,Monoclonal antibodies,Biosimilars,Mrna production,Regenerative medicine,Biopharmaceuticals,Blood and plasma,Oncology,Cell and gene therapy,Quality control,Infectious diseases,Bioprocessing
End products
Biologics,Biosimilar rituximab,Her2 targeted therapies,Anti-pd-1 monoclonal antibodies,Chemotherapeutic agents,Aav-based gene therapies,Mrna vaccines,Plasma-derived proteins,Influenza vaccines,Diagnostic kits,Car t-cell therapies,Regenerative skin grafts,Igg antibodies,Fc fusion proteins,Sars-cov-2 neutralizing antibodies
Steps before
Sample Preparation,Crude Sample Collection,Reagent Qualification,Method Development
Steps after
Data Analysis,Quality Control,Validation,Reporting,Sample Recovery
Input ingredients
proteins,antibodies,peptides,DNA,RNA,liposomes,viruses,virus-like particles (VLPs),serum,buffers containing DMSO,periplasmic fractions,untreated cell culture supernatants,crude cell lysates
Output ingredients
real-time kinetic binding sensorgrams,fitted result plots,residuals of fits,calibration curves,tabulated concentration data,tabulated kinetic data,epitope binning matrices,cross-blocking matrices,trace overlays
Market info
Sartorius AG is renowned for manufacturing high-precision laboratory and bioprocess equipment. They specialize in filtration, fermentation, and cell cultivation systems, known for their innovation and reliability in the pharmaceutical and biotech industries.

Technical Specifications


Number of Channels
8 independent channels
Simultaneous Sample Analysis
Up to 96 samples
Data Collection Rate
2,5 or 10 Hz
Sample Volume
180–220 µL/well
Temperature Control
15°C to 40°C
Spectrometers
8
Automation
GxP compliant options
Detection Technology
Bio-Layer Interferometry (BLI)
Molecular Weight Detection Limit
>150 Da
Association Rate Constant (ka)
10^1 to 10^7 M^-1s^-1
Dissociation Rate Constant (kd)
10^-6 to 10^-1 s^-1
Affinity Constant (KD)
1 mM – 10 pM
Baseline Noise (RMS)
<3.0 pm
Baseline Drift
<0.12 nm/hour
Instrument Weight
72 lb (32.7 Kg)
Instrument Dimensions (H x W x D)
19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm)
Analysis Time per Sample
5 minutes to 4 hours
Quantitation Time for Human IgG
2 minutes for 8 samples
Direct Quantitation Range
0.05 – 2000 μg/mL

Operating Characteristics


Automation level
Manual / PLC / SCADA
Batch vs. continuous operation
Batch / Inline Continuous
CIP/SIP
CIP 121°C / SIP 135°C
Energy efficiency
0.5–2 kWh/kg moisture
Unattended Run Time
Up to 12 hours
Sample Plate Cooling
15°C to 40°C

Material Compatibility


Density/particle size
0.5–2.5 g/cm³ / 50–1000 µm

Physical Characteristics


Machine Footprint
19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm)
Weight
72 lb (32.7 Kg)

Custom Options


Control panel type
Optional evaporation cover for sample protection
Integration possibilities
GxP-compliant software integration
Channel usage
Eight parallel,independent channels for flexibility
Sample plate cooling
Temperature control from 15-40°C
Fluidics-free design
Minimized assay time and maintenance
Software upgrade
Optional 21 CFR Part 11 software