Multiplex Rt-qpcr master mix for diagnostic development
Efficiently multiplex up to six targets with high sensitivity, even in complex sample matrices, enabling reliable detection and gene expression analysis with robust stability under varied workflow conditions.
Enables High-Throughput Multiplex Pathogen Detection
The TaqPath DuraPlex 1-Step RT-qPCR Master Mix from Applied Biosystems offers a robust solution for pharmaceutical manufacturers and clinical laboratories. Designed for high-throughput, this master mix allows for simultaneous detection of up to six targets, facilitating RNA and DNA pathogen assays across viral, bacterial, and fungal applications. With its 4X concentrated formula, this mix supports efficient multiplexing, crucial for producing diagnostic test kits and gene expression panels.
The master mix provides automation-friendly handling with benchtop stability over an eight-hour workday, integrating seamlessly into existing laboratory workflows. Notably, it maintains performance even in the presence of challenging PCR inhibitors, thanks to its unique formulation. This reliability ensures reproducibility and accuracy, as verified by its functional testing and ISO 13485 certification for consistent lot-to-lot performance.
Optimized for diverse conditions, it withstands up to eight freeze-thaw cycles and remains stable at room temperature for a week post-opening. This adaptability supports varied laboratory environments. Available with or without ROX dye, the master mix offers flexibility in assay development, all while meeting compliance with current good manufacturing practices (cGMP).
Benefits
- Enhances assay efficiency with support for high-throughput multiplexing of up to six targets.
- Increases detection reliability by overcoming PCR inhibitors in complex samples.
- Saves time and reduces manual intervention through automation-friendly, benchtop-stable handling.
- Maintains assay consistency and accuracy across various conditions with excellent freeze-thaw stability.
- Meets stringent regulatory standards with cGMP compliance, ensuring quality and reproducibility.
- Applications
- Gene expression profiling,Real time pcr,Virus detection,Pathogen detection,Diagnostic development,Diagnostic testing
- End products
- Pathogen detection panels,Multiplex pcr kits,Diagnostic test kits,Gene expression panels,Dna detection assays,Rna detection assays,Viral load assays
- Steps before
- Sample Preparation,RNA/DNA Extraction,Nucleic Acid Purification
- Steps after
- Data Analysis,Gene Expression Profiling,Viral/Pathogen Detection Reporting
- Input ingredients
- RNA samples,DNA samples,Viral pathogens,Bacterial pathogens,Fungal pathogens,Purified RNA,Purified DNA,Clinical sample matrices (e.g.,VTM,stool,blood,urine)
- Output ingredients
- qPCR results,Gene expression profiles,Multiplex PCR data,Ct values,Dynamic range consistency,Viral detection,Bacterial detection,Fungal detection
- Market info
- Thermo Fisher Scientific is renowned for providing innovative scientific instruments, laboratory equipment, and engineered-to-order solutions across various industries, esteemed for their high-quality products and expertise in life sciences, pharmaceuticals, and biotechnology sectors.
- Concentration
- 4X
- Reaction Speed
- Fast or Standard
- Stability
- Room temperature (24°C) for up to 1 week / 4°C for 1 month
- Freeze-Thaw Cycles
- 8 cycles without performance loss
- Capacity
- 5 mL,0.5 mL,10 mL
- Format
- Single-Tube
- Pipeline Compatibility
- Virus Detection,Pathogen Detection,Real Time PCR (qPCR),Gene Expression
- Temperature Conditions
- Store at –15 to –25°C
- Reaction Volume
- 1000 x 20 μL Reactions
- Detection Targets
- Up to 6 targets in a single reaction well
- Automation Compatibility
- Supports workflow automation
- Cross-Reactivity
- RNA and DNA detection
- Handling Viscosity
- Lower viscosity for efficient pipetting
- Fluorescent Dye
- ROX / No ROX options available
- Performance
- Ensures reproducible results with consistent lot-to-lot performance
- Linearity
- 8-log input range in a 5-plex assay
- Automation level
- Manual / PLC / SCADA
- Batch vs. continuous operation
- Batch / Inline Continuous
- CIP/SIP
- CIP 121°C / SIP 135°C
- Cleaning method
- CIP / Manual
- Energy efficiency
- 0.5–2 kWh/kg moisture
- Biological compatibility
- Compatible with PCR inhibitors
- Corrosive resistance (e.g. acids)
- Resistance to common PCR inhibitors
- Cleanability
- Compatible with standard laboratory cleaning protocols
- Density/particle size
- Function with various clinical sample matrices
- Multiplexing Capability
- Yes,with up to 5 or 6 targets
- Suitable Assays
- Real Time PCR,qPCR
- GMP Compliance
- Yes
- ISO Certification
- ISO 13485
- FDA Registration
- Yes
- Stability at room temperature
- Up to 1 week at 24°C
- Stability under refrigeration
- Up to 1 month at 4°C
- Formulation type
- 4X concentrated
- Vial volume
- 0.5 mL,5 mL,10 mL
- Integration possibilities
- Automation-friendly,liquid handling
- User handling
- Intuitive