Cell retention device for high-density perfusion processes
Effortlessly manage high-density cell cultures with advanced ultrasonic separation, optimizing continuous bioprocesses by facilitating efficient cell retention and seamless media exchange.
Facilitates Continuous Cell Separation and Retention
The Applikon BioSep from Getinge is an ultrasonic cell retention device designed for continuous, high-density perfusion processes in biopharmaceutical applications. Utilizing high-frequency resonant ultrasonic waves, it efficiently separates cells and automatically removes cell debris, reducing fouling and ensuring long-term operation. Ideal for producing monoclonal antibodies, recombinant proteins, and gene therapy products, the BioSep supports capacities from 1 L/day to 1000 L/day, with up to 99% separation efficiency depending on cell concentration and flow. The system integrates seamlessly with any bioreactor, offering flexible operation modes including continuous and semicontinuous strategies. Proven under cGMP conditions, it meets strict compliance standards and offers robust documentation to support regulated environments. The inclusion of a Clean-in-Place (CIP) system simplifies maintenance, while customizable configurations are available to suit specific production needs.
Benefits
- Maximizes cell yield with efficient separation, enhancing productivity.
- Minimizes maintenance downtime with non-fouling ultrasonic technology.
- Supports regulatory compliance in pharmaceutical production with cGMP-approved documentation.
- Reduces operational costs through automated cell debris removal.
- Ensures scalability from development to production, accommodating diverse batch sizes.
- Applications
- Bioprocess technology,Biopharmaceuticals,Continuous bioprocessing,Cell cultures
- End products
- Biologics,Cell therapy products,Gene therapy products,Viral vectors,Vaccines,Monoclonal antibodies,Enzymes,Recombinant proteins
- Steps before
- Bioreactor Preparation,Media Preparation
- Steps after
- Cell Concentration,Washing,Downstream Processing
- Input ingredients
- fresh media,viable cells,cell concentration
- Output ingredients
- spent media,cell debris,separated cells,cell/particle concentration
- Market info
- Getinge is known for providing innovative healthcare and life sciences products, specializing in medical technology, infection control, surgical workflows, and critical care solutions. They maintain a strong reputation for quality and technological advancement in these sectors.
- Maximum perfusion rate
- 1 L/day to 1000 L/day
- Minimum perfusion rate
- 0.1 L/day to 100 L/day
- Separation efficiency
- Up to 99%
- Minimum cell concentration
- 2 x 10^5 cells/ml
- Bioreactor connection
- 6 mm OD tube,12 mm OD tube
- Medium inlet
- 3 mm hosebarb to 0.5" TC
- Return line to bioreactor
- 3 mm hosebarb to 0.5" TC
- Perfusion outlet
- 3 mm hosebarb to 0.5" TC
- Weight
- 0.1 kg to 70 kg
- Resonator volume
- 0.7 ml to 1450 ml
- BioSep Controller Power
- 15 Watt to 1500 Watt
- BioSep Controller Dimensions
- 305x130x130 mm to 450x300x450 mm
- BioSep Controller Weight
- 3.5 kg to 17.6 kg
- Working mechanism
- High frequency resonant ultrasonic waves
- Batch vs. continuous operation
- Continuous perfusion
- Automation level
- Automatic removal of cell debris
- Cleaning method
- No fouling or blocking,suitable for long term operation
- Setup flexibility
- Direct mounting onto bioreactor head plate
- Scalability
- Full scalability between different sizes (1 L/day to 1000 L/day)
- Operation mode
- Continuous and semi-continuous operation
- Separation efficiency
- Up to 99%
- Density/particle size
- 0.5–2.5 g/cm³ / 50–1000 µm
- Bioreactor compatibility
- Any brand
- Machine footprint
- Varies with model
- Control panel type
- Mounted with my-Control
- Resonator volume
- 0.7 ml (1L model) - 1450 ml (1000L model)
- Weight
- 0.1 kg (1L model) - 70 kg (1000L model)
- Maximum perfusion rate
- 1 L/day - 1000 L/day
- Minimum perfusion rate
- 0.1 L/day - 100 L/day
- Bioreactor connection
- 6 mm OD tube - Mounted separately
- Medium inlet
- 3 mm hosebarb - 0.5” TC
- Return line to bioreactor
- 3 mm hosebarb - 0.5” TC
- Perfusion outlet
- 3 mm hosebarb - 0.5” TC
- Perfusion options
- 1 L/day,10 L/day,50 L/day,200 L/day,1000 L/day
- Mounting options
- Bioreactor head plate or separate mounting
- Compatibility
- Compatible with any brand of bioreactor
- Operation modes
- Standard,semicontinuous,cell recirculation strategies
- Resonator volume options
- 0.7 ml,7 ml,50 ml,290 ml,1450 ml
- Cell concentration range
- Minimum 2 x 105 cells/ml (depending on cell size)